| 货号 | 12362S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | DLAT (4A4-B6-C10) Mouse mAb recognizes endogenous levels of total DLAT protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Dihydrolipoamide acetyltransferase (DLAT) transfers an acetyl group from pyruvate to CoA to synthesize acetyl-CoA (1-4). This protein, also known as the E2 subunit of the pyruvate dehydrogenase complex (PDC-E2), has been implicated in the literature as the primary autoantigen in primary biliary cirrhosis (2-5). Antimitochondrial antibodies (AMAs) are likely formed when DLAT is exposed to the immune system in apoptotic cells of the bile duct (3,5). Research studies have shown that in some cases, cosmetics, NSAIDs, chewing gum, acetaminophen, and other compounds could trigger exposure of DLAT in sensitive individuals (3). The presence of AMAs is often detectable before disease diagnosis (4,5). Research studies have also shown that activation of the Toll-like receptor-3 (TLR-3) pathway is involved in the progression from a subclinical to clinical state (4). |
| 存放说明 | -20C |
| 计算分子量 | 69 |
Western blot analysis of extracts from various cell lines using DLAT (4A4-B6-C10) Mouse mAb. Western blot检测多种细胞提取物，使用抗体为DLAT (4A4-B6-C10) Mouse mAb鼠单抗。 | |
Immunoprecipitation of DLAT from HeLa cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or DLAT (4A4-B6-C10) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DLAT (4A4-B6-C10) Mouse mAb. 免疫沉淀分析HeLa细胞提取物DLAT， 使用Mouse (G3A1) mAb IgG1 Isotype Control #5415 (泳道 2) 或 DLAT (4A4-B6-C10) Mouse mAb 鼠单抗(泳道3)。泳道1是10%对照。使用 DLAT (4A4-B6-C10) Mouse mAb鼠单抗进行Western blot检测。 | |
Confocal immunofluorescent analysis of HeLa cells using DLAT (4A4-B6-C10) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 共聚焦免疫荧光分析HeLa 细胞，使用抗体为DLAT (4A4-B6-C10) Mouse mAb 鼠单抗(绿色) ，DY-554 phalloidin (红色) 标记肌动蛋白纤丝。 蓝色伪彩=DRAQ5®＃4084（荧光DNA染料）。 |
