| 货号 | 12844S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DNAJC2/MPP11 (D6B1E) Rabbit mAb recognizes endogenous levels of total DNAJC2/MPP11 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | DnaJ/Hsp40 proteins are a conserved family of J-domain-containing chaperone proteins that assist in protein folding and stability through their interactions with Hsp70 chaperone proteins (reviewed in 1). DNAJC2, also known as MPP11 (M-phase phosphoprotein 11 protein), is a component of the ribosome-associated complex (RAC). The RAC is localized to the cytoplasm, where it assists in maintaining appropriate folding of nascent polypeptides by stimulating the ATPase activity of Hsp70 chaperone proteins (2,3). In the nucleus, MPP11 is involved in the activation of transcription through mediation of the switch from polycomb-repressed to active chromatin (4). Previous studies have shown MPP11 is overexpressed in leukemia and head and neck cancer, leading researchers to suggest MPP11 may be a potential therapeutic target (5-7). |
| 存放说明 | -20C |
| 计算分子量 | 80 |
Western blot analysis of extracts from various cell lines using DNAJC2/MPP11 (D6B1E) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 DNAJC2/MPP11 (D6B1E) Rabbit mAb兔单抗。 | |
Immunoprecipitation of DNAJC2/MPP11 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DNAJC2/MPP11 (D6B1E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using DNAJC2/MPP11 (D6B1E) Rabbit mAb. 免疫沉淀法检测 MCF7细胞提取物中的DNAJC2/MPP11，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2) 或 DNAJC2/MPP11 (D6B1E) Rabbit mAb 兔单抗(泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是DNAJC2/MPP11 (D6B1E) Rabbit mAb兔单抗。 |
