| 货号 | 12311S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-IC |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | DNA-dependent protein kinase (DNA-PK) is an important factor in the repair of double-stranded breaks in DNA. Cells lacking DNA-PK or in which DNA-PK is inhibited fail to show proper nonhomologous end-joining (NHEJ) (1-7). DNA-PK is composed of two DNA-binding subunits (Ku70 and Ku86) and one 450 kDa catalytic subunit (DNA-PKcs) (8). It is thought that a heterodimer of Ku70 and Ku86 binds to double-stranded DNA broken ends before DNA-PKcs binds and is activated (1,9). Activated DNA-PKcs is a serine/threonine kinase that has been shown to phosphorylate a number of proteins in vitro, including p53, transcription factors, RNA polymerase, and Ku70/Ku86 (10,11). DNA-PKcs autophosphorylation at multiple sites, including Thr2609 and Ser2056, results in an inactivation of DNA-PK kinase activity and NHEJ ability (12,13). It has been demonstrated, however, that DNA-PK preferentially phosphorylates substrates before it autophosphorylates, suggesting that DNA-PK autophosphorylation may play a role in disassembly of the DNA repair machinery (14,15). Autophosphorylation at Thr2609 has also been shown to be required for DNA-PK-mediated double strand break repair, and phosphorylated DNA-PK co-localizes with H2A.X and 53BP1 at sites of DNA damage (16). Phosphorylation at Ser2056 occurs in response to double-stranded DNA breaks and ATM activation (17).DNA依赖蛋白激酶（DNA-PK）是DNA双链损伤修复过程中的重要因子。缺少DNA-PK或DNA-PK被抑制的细胞将无法进行正常的非同源末端连接(NHEJ) (1-7).DNA-PK包含了两个DNA结合亚基（Ku70和Ku86）以及1个450kDa的催化亚基(DNA-PKcs) (8). Ku70和Ku86的异二聚体可以在DNA-PKcs结合前和双链DNA损伤末端结合并激活（1,9）。激活的DNA-PK是一个丝氨酸/苏氨酸激酶可以磷酸化一系列的蛋白，包括p53，转录因子，RNA聚合酶和Ku70/Ku86（10,11）。DNA-PKcs可以在多个位点自磷酸化，包括2609位苏氨酸和2056位丝氨酸，将导致DNA-PK的激酶活性和NHEJ能力丢失（12,13）。但是研究已经证明，DNA-PK在进行自磷酸化前会选择性的磷酸化其底物，这提示了DNA-PK的自磷酸化可能在DNA损伤修复机制中发挥了作用（14,15）。已经发现2609位苏氨酸自磷酸化对DNA-PK介导的双链损伤修复，磷酸化的DNA-PK与H2A.X 和53BP1的共定位在DNA损伤的位点都是必须的（16）。2056位丝氨酸的磷酸化是对双链DNA损伤和ATM激活的应激（17）。 |
| 存放说明 | -20C |
| 计算分子量 | 450 |
Confocal immunofluorescent analysis of M059K (DNA-PK wild-type; left) and M059J (DNA-PK deficient; right) cells using DNA-PK (3H6) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).使用DNA-PK (3H6)Mouse mAb（绿色）对M059K（野生型DNA-PK；左）和M059J（缺陷型DNA-PK；右）进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DY-554鬼笔环肽标记（红色）。 | |
Immunohistochemical analysis of paraffin-embedded cell pellets, M059K (DNA-PK wild-type; left) or M059J (DNA-PK deficient; right), using DNA-PK (3H6) Mouse mAb.使用DNA-PK (3H6)Mouse mAb对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using DNA-PK (3H6) Mouse mAb.使用DNA-PK (3H6)Mouse mAb对M059K（野生型DNA-PK；左）和M059J（缺陷型DNA-PK；右）提取物进行western blot分析。 |
