| 货号 | 13451S |
| 反应种属 | Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) recognizes endogenous levels of total DNMT3L protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Western blot analysis of extracts from F9 and mES cells using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific).Western blot方法检测F9和mES细胞系的提取物，使用的抗体为DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific)。 | |
Immunoprecipitation of DNMT3L from F9 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (lane 3). Lane 1 is 10% input. Western blot analysis was performed using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific).从F9细胞提取物中免疫沉淀DNMT3L，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (lane 3)。Lane 1为10% input。 使用DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific)进行Western blot检测。 | |
Western blot analysis of extracts from F9 cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® DNMT3L siRNA I (Mouse Specific) #12307 (+), or SignalSilence® DNMT3L siRNA II (Mouse Specific) #12308 (+), using DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower). The DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) confirms silencing of DNMT3L expression, while the GAPDH (D16H11) XP® Rabbit mAb is used as a loading control.Western blot方法检测经100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-), SignalSilence® DNMT3L siRNA I (Mouse Specific) #12307 (+), 或SignalSilence® DNMT3L siRNA II (Mouse Specific) #12308 (+) 转染的F9细胞系提取物，使用的抗体为DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) (上图) 或GAPDH (D16H11) XP® Rabbit mAb #5174 (下图)。使用DNMT3L (E1Y7Q) Rabbit mAb (Mouse Specific) 确认DNMT3L 表达的沉默效率， GAPDH (D16H11) XP®Rabbit mAb用作上样量对照。 |
