| 货号 | 4846S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DOCK180 (C4C12) Rabbit mAb recognizes endogenous levels of total DOCK180 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | DOCK180 and its partner, ELMO1, interact directly with one another to form an atypical two-part guanine nucleotide exchange factor (GEF) for the small GTPase Rac (1). Rac activation occurs in association with p130 Cas and Crk, which form a complex with DOCK180 that is targeted to focal adhesions (2,3). DOCK180 is also recruited to the plasma membrane by binding to phosphoinositides (4). ELMO1 may function as an inhibitor of proteasome-dependent degradation of DOCK180 at the plasma membrane to regulate reorganization of the actin cytoskeleton (5). Localized Rac activation allows actin nucleation via WAVE family proteins, signaling to integrins, formation of lamellipodia and filopodia, and regulation of processes such as phagocytosis and cell migration (6-8). |
| 存放说明 | -20C |
| 计算分子量 | 215 |
Western blot analysis of extracts from various cell types, using DOCK180 (C4C12) Rabbit mAb. 使用DOCK180 (C4C12) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中DOCK180 (C4C12)蛋白水平。 | |
Immunoprecipitation of DOCK180 from HCT15 cells, using DOCK180 (C4C12) Rabbit mAb. Western blot was performed using the same antibody. 使用DOCK180 (C4C12) Rabbit mAb，免疫沉淀（Immunoprecipitation）分析HCT15细胞的DOCK180蛋白。使用同样的抗体进行免疫印迹（Western blot）实验。 |
