| 货号 | 67138S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | DDP4 (CD26) is a type II transmembrane glycoprotein expressed ubiquitously in most tissues and different cell types (1,2). The protein has a short cytoplasmic domain, transmembrane domain, a flexible stalk fragment and extracellular fragment (2). Both the catalytic peptide hydrolase domain and the beta-propeller ligand binding domain are located in the extracellular fragment (2). DDP4 is a multifunctional protein that exists in both a membrane bound form as well as an extracellular soluble form. As a peptidase, it removes N-terminal dipeptides sequentially from proteins with a proline or alanine as the penultimate P1 amino acid (3.4). DPP4 has been shown to cleave a wide range of substrates including GLP-1, BNP, substance P, etc. It is also involved in the regulation of related biological functions (5). In addition to it peptidase activity, DDP4 interacts with multiple important cell surface ligands, such as adenosine deaminase, fibronectin, and IGF2 receptor to influence processes like T cell activation, cell migration and proliferation (5). Several DDP4 inhibitors have been developed and their effects have been tested in the field of diabetes, cardiovascular disease and tumor immunity (2,5,6). |
| 存放说明 | -20C |
| 计算分子量 | 90, 120 |
Confocal immunofluorescent analysis of Caco-2 (positive, left) and HT-1080 (negative, right) cells using DPP4 (D6D8K) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from Lovo, Caco-2 and IGROV-1 cell lines using DPP4 (D6D8K) Rabbit mAb. | |
Immunoprecipitation of DPP4 protein from LoVo cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is DPP4 (D6D8K) Rabbit mAb. Western blot analysis was performed using DPP4 (D6D8K) Rabbit mAb. |
