| 货号 | 8570S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | DRP1 (D6C7) Rabbit mAb recognizes endogenous levels of total DRP1 protein. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:50) |
| 供应商 | CST |
| 背景 | Dynamin-related protein 1 (DRP1) is a member of the dynamin superfamily of GTPases. Members of this family have diverse cellular functions including vesicle scission, organelle fission, viral resistance, and intracellular trafficking (reviewed in 1). DRP1 affects mitochondrial morphology and is important in mitochondrial and peroxisomal fission in mammalian cells (2-5). The yeast ortholog of DRP1 clusters into a spiral-shaped structure on the mitochondrial membrane at the site of fission (reviewed in 6), and this structure is likely conserved in mammalian cells (3). The division of the mitochondria, which is required for apoptosis as well as normal cell growth and development, is controlled, in part, by the phosphorylation of DRP1 at Ser616 by Cdk1/cyclin B and at Ser637 by protein kinase A (PKA) (reviewed in 6). When phosphorylated at Ser616, DRP1 stimulates mitochondrial fission during mitosis. Conversely, fission is inhibited when DRP1 is phosphorylated at Ser637 (reviewed in 6). Dephosphorylation of Ser637 by calcineurin reverses this inhibition (7). In addition to phosphorylation, sumoylation of DRP1 is also an enhancer of mitochondrial fission (8). Balancing fission and fusion events is essential for proper mitochondrial function. Mitochondrial defects are seen in a variety of neurodegenerative diseases including Alzheimers disease, Parkinsons disease, and Huntingtons disease (reviewed in 6). |
| 存放说明 | -20C |
| 计算分子量 | 78-82 |
Confocal immunofluorescent analysis of ACHN cells using DRP1 (D6C7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用DRP1 (D6C7) Rabbit mAb (绿色)标记，共聚焦免疫荧光分析ACHN细胞。DY-554 phalloidin标记微丝蛋白（红色）。蓝色= DRAQ5® #4084（DNA荧光染料）。 | |
Western blot analysis of extracts from various cell lines using DRP1 (D6C7) Rabbit mAb.使用DRP1 (D6C7) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中DRP1 (D6C7)蛋白水平。 | |
Immunoprecipitation of extracts from HCT 116 cells using DRP1 (D6C7) Rabbit mAb. The western blot was probed using the same antibody. Lane 1 is 10% input.使用DRP1 (D6C7) Rabbit mAb，免疫沉淀（Immunoprecipitation）分析HCT 116细胞。使用同样的抗体进行免疫印迹（Western blot）实验。第1道是10% input。 |
