| 货号 | 5672S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | DYRK1B (D40D1) Rabbit mAb recognizes endogenous levels of total DYRK1B protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | The DYRK family includes several dual-specificity tyrosine-phosphorylated and regulated kinases capable of phosphorylating proteins at both tyrosine and serine/threonine residues (1). The DYRK family was identified based on homology to the yeast Yak1 (2) and the Drosophila minibrain (mnb) kinases (3). Seven mammalian isoforms have been discovered, including DYRK1A, DYRK1B, DYRK1C, DYRK2, DYRK3, DYRK4, and DYRK4B. Differences in substrate specificity, expression, and subcellular localization are seen across the DYRK family (4,5). All DYRK proteins have a Tyr-X-Tyr motif in the catalytic domain activation loop, with phosphorylation of the second Tyr residue (e.g. Tyr312 of DYRK1A) necessary for kinase activity. DYRKs typically autophosphorylate the tyrosine residue within their activation loop, but phosphorylate substrates at serine and threonine residues (1,6).In contrast to the ubiquitous DYRK1A, DYRK1B exhibits relatively restricted expression with highest levels found in the testis and muscle (7,8). Three major DYRK1B splice variants demonstrate distinct expression patterns and functional properties (9). DYRK1B plays a critical role in myoblast differentiation by affecting cell motility, transcription, cell cycle progression, and survival (10,11). In addition, DYRK1B contributes to the survival of certain cancer cells (7,12,13). |
| 存放说明 | -20C |
| 计算分子量 | 70-80 |
Western blot analysis of extracts from various cell lines and tissues using DYRK1B (D40D1) Rabbit mAb. Western blot分析多个细胞系和组织提取物，使用的抗体是DYRK1B (D40D1) Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a human DYRK1B contstruct (+), using DYRK1B (D40D1) Rabbit mAb. Western blot分析293T细胞提取物，空载体转染(-)或转染人DYRK1B载体(+)，使用的抗体是DYRK1B (D40D1) Rabbit mAb 兔单抗。 |
