| 货号 | 5166S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 使用方法 | WB(1:1000) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | Regulation of the E2F family of transcription factors, primarily through the retinoblastoma protein (pRb), is vital for control of cell proliferation and cell death (reviewed in 1). E2F-associated phosphoprotein (EAPP) was identified as an E2F-family binding protein that modulates E2F-regulated transcription and may be required for S phase entry. EAPP is expressed at varied levels in all tissues and cell types examined and its expression is reduced in nocodazole-arrested cells (2). Mass spectrometry studies have identified multiple EAPP phosphorylation sites including Ser109 and Ser111, but biological consequences of EAPP phosphorylation have yet to be elucidated (3-5).E2F转录因子家族的调节主要通过pRb，该过程对于细胞增殖和细胞死亡至关重要(回顾1)。E2F相关的磷蛋白(EAPP)被鉴定为E2F家族结合蛋白，能够调节E2F调控的转录并可能参与S期的进入。EAPP在所有的组织和细胞中表达各不相同，而且其水平在诺考达唑捕获的细胞中是降低的(2)。质谱研究已经鉴定出EAPP的多个磷酸化位点包括109位和111位的丝氨酸，但是EAPP磷酸化的生物学作用还未被阐明(3-5)。 |
| 存放说明 | -20C |
| 计算分子量 | 45 |
Western blot analysis of extracts from various cell types using EAPP (1E4) Mouse mAb.Western blot方法检测不同细胞提取物，使用的抗体为EAPP (1E4) Mouse mAb。 | |
Confocal immunofluorescent analysis of MCF7 cells using EAPP (1E4) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).激光共聚焦免疫荧光方法检测MCF7细胞，使用的抗体为 EAPP (1E4) Mouse mAb，为绿色。肌动蛋白纤维由DY-554鬼笔环肽标记，呈红色。蓝色伪彩为DNA荧光染料（产品信息为DRAQ5® #4084 ）。 |
