| 货号 | 3195S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. |
| 使用方法 | WB(1:1000) IHC-P (1:400) F (1:200) IF-IC (1:200) IHC-F (1:200) |
| 供应商 | CST |
| 背景 | Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with β-catenin, γ-catenin (also called plakoglobin), and p120 catenin. β-catenin and γ-catenin associate with α-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). While β- and γ-catenin play structural roles in the junctional complex, p120 regulates cadherin adhesive activity and trafficking (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch". N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 135 |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).在对照肽（左）或E-Cadherin 封闭肽#1050（右）存在条件下，使用E-Cadherin (24E10) 兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb. | |
Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.使用E-Cadherin (24E10) 兔单抗对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.使用E-Cadherin (24E10) 兔单抗对石蜡包埋的人淋巴结转移性恶性腺瘤组织进行免疫组化分析。 | |
Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).使用E-Cadherin (24E10) 兔单抗（绿色，左）对比异型对照（右）对MCF7细胞进行激光共聚焦免疫荧光分析。蓝色假色=DRAQ5®（DNA荧光染料）。 | |
Immunohistochemical analysis of frozen HCC827 xenograft, showing membrane and cytoplasmic localization using E-Cadherin (24E10) Rabbit mAb.使用E-Cadherin (24E10) 兔单抗对冰冻的HCC827移植物进行免疫组化分析，显示细胞膜和细胞质位置。 | |
Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.使用使用E-Cadherin (24E10) 兔单抗对石蜡包埋的小鼠肺组织进行免疫组化分析。 | |
Flow cytometric analysis of HeLa cells (blue) and MCF7 cells (green) using E-Cadherin (24E10) Rabbit mAb.使用E-Cadherin (24E10) 兔单抗对HeLa细胞（蓝色）和MCF7细胞（绿色）进行流式细胞分析。 |
