| 货号 | 4203S |
| 反应种属 | Human |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 使用方法 | WB(1:2000) IP (1:100) |
| 供应商 | CST |
| 背景 | Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5).Eg5(也称作驱动蛋白样蛋白 11或者Kif11)属于马达蛋白的驱动蛋白样家族，在染色体定位、中心体分离和有丝分裂纺锤体形成过程中起重要作用。有丝分裂激酶磷酸化Eg5能够通过它与微管的关系调节其活性(1,2)。由于抗有丝分裂化疗药物如紫杉醇，靶向微管并具有多效性，有时甚至是毒性作用，现在靶向微管相关蛋白药物如Eg5正在开发中(3-5)。 |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts form HeLa and 293 cells using Eg5 (4H3-1F12) Mouse mAb.Western blot方法检测HeLa 和 293细胞提取物，使用的抗体为 Eg5 (4H3-1F12) Mouse mAb。 | |
Immunoprecipitation of Eg5 from HeLa cell extracts using Eg5 (4H3-1F12) Mouse mAb followed by western blot using the same antibody. IP (-) is 5% input.从HeLa细胞提取物中免疫沉淀Eg5，使用的抗体为Eg5 (4H3-1F12) Mouse mAb，随后使用同一抗体进行western blot检测。IP(-)是5% input。 |
