| 货号 | 14404S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Eg5 (also called kinesin-like protein 11 or Kif11) belongs to the kinesin-like family of motor proteins important in chromosome positioning, centrosome separation, and mitotic spindle formation. Phosphorylation of Eg5 by mitotic kinases regulates its activity by modulating its association with microtubules (1,2). Because anti-mitotic chemotherapeutic drugs, such as taxanes, target microtubules and have pleiotropic and sometimes toxic effects, drugs that target microtubule-associated proteins such as Eg5 are currently in development (3-5). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Confocal immunfluorescent analysis of 293T cells using Eg5 (E1L3W) Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from A-431, MCF7, and COLO 205 cells using Eg5 (E1L3W) Rabbit mAb. | |
Immunoprecipitation of Eg5 from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Eg5 (E1L3W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Eg5 (E1L3W) Rabbit mAb. |
