| 货号 | 12496S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | eIF1 (D7G3L) Rabbit mAb recognizes endogenous levels of total eIF1 protein. Based upon sequence alignment, this antibody may cross-react with eIF1B. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Eukaryotic translation initiation factor 1 (eIF1) was first purified as a factor stimulating binding of Met-tRNA and mRNA to the ribosome (1,2). eIF1 is essential for growth in yeast and two classes of mutations in yeast eIF1 indicate a role for this protein in ensuring accurate translation initiation site selection (3). It has been demonstrated that eIF1 expression is stress-inducible, suggesting that modulation of translation initiation occurs during cellular stress (4). |
| 存放说明 | -20C |
| 计算分子量 | 13 |
Western blot analysis of extracts from various cell lines using eIF1 (D7G3L) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 eIF1 (D7G3L) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human eIF1 (heIF1-Myc/DDK; +), using eIF1 (D7G3L) Rabbit mAb. Western blot方法检测293T细胞提取物，细胞分别用空白对照物（-）或构建的带有Myc/DDK标签的全长人eIF1 (heIF1-Myc/DDK; +)转染，使用的抗体为eIF1 (D7G3L) Rabbit mAb兔单抗。 | |
Immunoprecipitation of eIF1 from 293T cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or eIF1 (D7G3L) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using eIF1 (D7G3L) Rabbit mAb. 免疫沉淀法检测 293T细胞提取物中的 eIF1，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或 eIF1 (D7G3L) Rabbit mAb (泳道3)。Lane 1为10% input对照。 Western blot 检测中使用的是 eIF1 (D7G3L) Rabbit mAb兔单抗。 |
