| 货号 | 2103S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P |
| 目标/特异性 | eIF2α (L57A5) Mouse mAb detects endogenous levels of total eIF2α protein. |
| 使用方法 | WB(1:1000) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | Phosphorylation of the eukaryotic initiation factor 2 (eIF2) α subunit is a well-documented mechanism to downregulate protein synthesis under a variety of stress conditions. Eukaryotic initiation factor 2 binds GTP and Met-tRNAi and transfers Met-tRNA to the 40S subunit to form the 43S preinitiation complex (1,2). eIF2 promotes a new round of translation initiation by exchanging GDP for GTP, a reaction catalyzed by eIF2B (1,2). Kinases that are activated by viral infection (PKR), endoplasmic reticulum stress (PERK/PEK), amino acid deprivation (GCN2), or heme deficiency (HRI) can phosphorylate the α subunit of eIF2 (3,4). This phosphorylation stabilizes the eIF2-GDP-eIF2B complex and inhibits the turnover of eIF2B. Induction of PKR by IFN-γ and TNF-α induces potent phosphorylation of eIF2α at Ser51 (5,6). |
| 存放说明 | -20C |
| 计算分子量 | 38 |
Western blot analysis of extracts from various cell types using eIF2α (L57A5) Mouse mAb. 使用eIF2α (L57A5) Mouse mAb鼠单抗，免疫印迹(Western Blotting)分析不同细胞系提取物。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF2α (L57A5) Mouse mAb. 使用eIF2α (L57A5) Mouse mAb鼠单抗，免疫组化分析人类结肠癌组织石蜡切片 。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF2α (L57A5) Mouse mAb. 使用eIF2α (L57A5) Mouse mAb鼠单抗，免疫组化分析人类肺癌组织石蜡切片。 |
