| 货号 | 2067S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | eIF4E (C46H6) Rabbit mAb detects endogenous levels of total eIF4E protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:1600) |
| 供应商 | CST |
| 背景 | Eukaryotic initiation factor 4E (eIF4E) binds to the mRNA cap structure to mediate the initiation of translation (1,2). eIF4E interacts with eIF4G, a scaffold protein that promotes assembly of eIF4E and eIF4A into the eIF4F complex (2). eIF4B is thought to assist the eIF4F complex in translation initiation. Upon activation by mitogenic and/or stress stimuli mediated by Erk and p38 MAPK, Mnk1 phosphorylates eIF4E at Ser209 in vivo (3,4). Two Erk and p38 MAPK phosphorylation sites in mouse Mnk1 (Thr197 and Thr202) are essential for Mnk1 kinase activity (3). The carboxy-terminal region of eIF4G also contains serum-stimulated phosphorylation sites, including Ser1108, Ser1148, and Ser1192 (5). Phosphorylation at these sites is blocked by the PI3 kinase inhibitor LY294002 and by the FRAP/mTOR inhibitor rapamycin. |
| 存放说明 | -20C |
| 计算分子量 | 25 |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using eIF4E (C46H6) Rabbit mAb. 使用eIF4E (C46H6) Rabbit mAb兔单抗，免疫组化分析人类结肠癌组织的石蜡切片。 | |
Western blot analysis of extracts from various cell lines using eIF4E (C46H6) Rabbit mAb. 使用eIF4E (C46H6) Rabbit mAb兔单抗，免疫印迹（Western Blotting）分析不同细胞系中提取物。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4E (C46H6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 使用eIF4E (C46H6) Rabbit mAb兔单抗，免疫组化分析人类乳腺癌组织的石蜡切片，其分别孵育对照多肽（左图）和抗原特异的多肽 (右图)。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® eIF4E siRNA I #6311 or SignalSilence® eIF4E siRNA II #6554 (+), using eIF4E (C46H6) Rabbit mAb #2067 and α-Tubulin (11H10) Rabbit mAb #2125. The eIF4E (C46H6) Rabbit mAb confirms silencing of eIF4E expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of eIF4E siRNA. 使用eIF4E (C46H6) Rabbit mAb兔单抗#2067 和 α-Tubulin (11H10) Rabbit mAb兔单抗 #2125，免疫印迹（Western Blotting）分析HeLa细胞系提取物。分为转染100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-)组，转染gnalSilence® eIF4E siRNA I #6311组和SignalSilence®eIF4E siRNA II #6554 (+)组。eIF4E (C46H6) Rabbit mAb兔单抗确证eIF4E基因表达沉默，而α-Tubulin (11H10) Rabbit mAb兔单抗用于蛋白的上样对照和确定eIF4E siRNA特异性。 |
