| 货号 | 8701S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | eIF4GI (D6A6) Rabbit mAb recognizes endogenous levels of total eIF4GI protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5 m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8). |
| 存放说明 | -20C |
| 计算分子量 | 220 |
Western blot analysis of extracts from various cell lines using eIF4GI (D6A6) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为eIF4GI (D6A6) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcioma using eIF4GI (D6A6) Rabbit mAb in the presence of control peptide (left) or antigen-specifc peptide (right). 免疫组化分别检测在对照多肽（左）或抗原特异性多肽（右）存在的情况下石蜡包埋的人结肠肿瘤，所使用的抗体为 eIF4GI (D6A6) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4GI (D6A6) Rabbit mAb. 免疫组化检测石蜡包埋的人乳腺肿瘤，所使用的抗体为 eIF4GI (D6A6) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4GI (D6A6) Rabbit mAb. 免疫组化检测石蜡包埋的人肺肿瘤，所使用的抗体为 eIF4GI (D6A6) Rabbit mAb兔单抗。 |
