| 货号 | 12582S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | ELAVL1/HuR (D9W7E) Rabbit mAb recognizes endogenous levels of total ELAVL1/HuR protein. |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3 UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Confocal immunofluorescent analysis of NIH/3T3 cells, untreated (left) or actinomycin D-treated (5 μg/ml, 2 hr; right) using ELAVL1/HuR (D9W7E) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 采用共聚焦免疫荧光术分别检测未处理（左）或actinomycin D处理 (5 μg/ml, 2小时; 右)的 NIH/3T3细胞，使用的抗体为 ELAVL1/HuR (D9W7E) Rabbit mAb兔单抗(绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。 | |
Western blot analysis of extracts from various cell lines using ELAVL1/HuR (D9W7E) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 ELAVL1/HuR (D9W7E) Rabbit mAb兔单抗。 | |
Immunoprecipitation of ELAVL1/HuR from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ELAVL1/HuR (D9W7E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ELAVL1/HuR (D9W7E) Rabbit mAb. 免疫沉淀法检测293细胞提取物中的ELAVL1/HuR，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或 ELAVL1/HuR (D9W7E) Rabbit mAb (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是 ELAVL1/HuR (D9W7E) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human ELAVL1/HuR (hELAVL1/HuR-Myc/DDK; +), using ELAVL1/HuR (D9W7E) Rabbit mAb (upper), DYKDDDDK Tag (9A3) Mouse mAb #8146 (middle), or β-Actin (D6A8) Rabbit mAb #8457 (lower). Western blot方法检测293细胞提取物，细胞分别使用空白对照物（-）或构建的Myc/DDK标签的全长人ELAVL1/HuR (hELAVL1/HuR-Myc/DDK; +)转染，使用的抗体为 ELAVL1/HuR (D9W7E) Rabbit mAb兔单抗 (上图), DYKDDDDK Tag (9A3) Mouse mAb鼠单抗 #8146 (中),或 β-Actin (D6A8) Rabbit mAb兔单抗 #8457 (下图)。 |
