| 货号 | 12548S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:2000) |
| 供应商 | CST |
| 背景 | <p>Echinoderm microtubule-associated protein-like 4 (EML4) is a 120 kDa microtubule-associated WD-repeat protein of the echinoderm microtubule-associated protein family (1). The expression of EML4 is necessary for correct intracellular microtubule network formation (2). EML4 protein expression is upregulated during mitosis and downregulated during the remaining cell cycle (1). During mitosis, EML4 is heavily phosphorylated and is associated with the mitotic spindle (2). The amino-terminal segment of EML4 is essential for microtubule association and function.</p> 棘皮动物微管样蛋白4（EML4）是棘皮动物微管样蛋白家族一个120kDa的微管相关的WD重复蛋白（1）。EML4的表达对于正确的细胞内微管网络形成是必须的（2）。有丝分裂时EML4表达上升，在静息细胞时期表达下降（1）。有丝分裂时，EML4被深度磷酸化且和有丝分裂纺锤体结合（2）。EML4的氨基末端序列对于微管的结合和功能是必须的。 |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using EML4 (D5V6M) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).在对照肽（左）或抗原特异性肽（右）存在的条件下，使用EML4 (D5V6M) Rabbit mAb对石蜡包埋的人肺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using EML4 (D5V6M) Rabbit mAb.使用EML4 (D5V6M) Rabbit mAb对石蜡包埋的人前列腺癌组织进行免疫组化分析。 | |
Western blot analysis of extracts from various cell lines using EML4 (D5V6M) Rabbit mAb.使用EML4 (D5V6M) Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunoprecipitation of EML4 from 293 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or EML4 (D5V6M) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EML4 (D5V6M) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或EML4 (D5V6M) Rabbit mAb (lane 3)对293细胞的EML4进行免疫沉淀实验。Lane1是10%上样量。使用EML4 (D5V6M) Rabbit mAb进行western blot分析。 |
