| 货号 | 12460S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | Eps15 (D3K8R) Rabbit mAb recognizes endogenous levels of total Eps15 protein. Based upon sequence alignment, this antibody is predicted to react with both Eps15a and Eps15b. This antibody does not cross-react with Eps15R. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Eps15 (EGFR pathway substrate 15) was originally discovered as a substrate for the kinase activity of EGFR (1). Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionarily conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet Asp-Pro-Phe that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin (1). Several lines of evidence support a role for Eps15 in clathrin-mediated endocytosis, including the endocytosis of synaptic vesicles. Eps15 binds to AP2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles (2). Eps15 regulates the endosomal trafficking of c-Met (3) and EGFR (4), possibly by recruiting the ubiquitinated receptors to the rims of clathrin-coated pits through interaction between the ubiquitin tag and its UIMs. The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and are thus implicated in different facets of endosomal trafficking (5). Human EPS15 has been mapped to chromosome 1p31-p32, a region displaying several nonrandom chromosomal abnormalities, including deletions in neuroblastoma and translocations in acute lymphoblastic and myeloid leukemias. Research has shown two translocations t(1;11)(p32;q11) are found in rare cases of myeloid leukemia where the Eps15 gene was fused to the HRX gene, resulting in two reciprocal fusion genes (6). |
| 存放说明 | -20C |
| 计算分子量 | 138 |
Western blot analysis of extracts from various cell lines using Eps15 (D3K8R) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为 Eps15 (D3K8R) Rabbit mAb兔单抗。 | |
Immunoprecipitation of Eps15 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Eps15 (D3K8R) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Eps15 (D3K8R) Rabbit mAb. 免疫沉淀法检测MCF7细胞提取物中的Eps15 ，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 （泳道2）或 Eps15 (D3K8R) Rabbit mAb兔单抗 (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是 Eps15 (D3K8R) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of A-431 cells, serum-starved (left) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/mL, 1 hr, 4ºC; right), using Eps15 (D3K8R) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 采用共聚焦免疫荧光术分别检测血清饥饿（左图）或人表皮生长因子(hEGF) #8916（100 ng/mL, 1小时, 4ºC;右图）处理的A-431细胞，使用的抗体为Eps15 (D3K8R) Rabbit mAb兔单抗 (绿色)。蓝色伪彩= DRAQ5® #4084 (荧光DNA染料)。 |
