| 货号 | 12048S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | EREG (D4O5I) Rabbit mAb recognizes endogenous levels of total EREG protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Epiregulin (EREG) belongs to the epidermal growth factor (EGF) family and is a ligand for the EGF receptor (EGFR) and ErbB4 (1-3). The binding of EREG to homodimers, as well as heterodimers containing EGFR or ErbB4, leads to receptor activation and downstream signaling to promote cell growth and proliferation (4-6). In normal human tissue, moderate levels of EREG are expressed in the placenta and peripheral blood macrophages. Research studies have shown that EREG is expressed at high levels in numerous cancer cell lines, and EREG expression is correlated with primary cancer aggresiveness/metastases (7-11). In addition to its involvement in tumorigenesis, a variant of EREG has also been shown to be associated with tuberculosis susceptibility (12). EREG migrates as multiple bands including a ~30 kDa glycosylated membrane bound form (proepiregulin), a 19 kDa nonglycosylated propeptide form, and a 17 kDa mature form (13). |
| 存放说明 | -20C |
| 计算分子量 | 17,19, 30 |
Western blot analysis of extracts from various cell lines using EREG (D4O5I) Rabbit mAb.Western blot方法检测多个细胞系的提取物，使用的抗体为EREG (D4O5I) Rabbit mAb. | |
Immunoprecipitation of EREG from COLO 205 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or EREG (D4O5I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using EREG (D4O5I) Rabbit mAb.免疫沉淀方法检测COLO细胞提取物，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (泳道2)或EREG (D4O5I) Rabbit mAb（泳道3）。泳道1是加入10%蛋白。用EREG (D4O5I) Rabbit mAb进行Western blot检测。 |
