| 货号 | 14069S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IF-IC/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) IF-IC (1:3200) ChIP (1:50) |
| 供应商 | CST |
| 背景 | ETS-1 is a proto-oncoprotein that belongs to the E26 Transformation-specific Sequence (ETS) family of transcription factors that share a unique and highly conserved DNA binding domain (1). ETS-1 plays important roles in vascular development and angiogenesis (2), and vascular inflammation and remodeling (3). The target genes of ETS-1 include receptor tyrosine kinases, MMPs, and cell adhesion molecules (4-6). In addition, ETS-1 is involved in regulation of energy metabolism in cancer cells (7). ETS-1 activity is regulated by two different types of phosphorylation sites. While phosphorylation at a cluster of serine residues in the exon VII domain by CaMKII inhibits ETS-1 DNA binding activity (8), phosphorylation at its Thr38 site by Ras activates ETS-1 (9). |
| 存放说明 | -20C |
| 计算分子量 | 52 |
Confocal immunofluorescent analysis of OVCAR8 (high expression, left), A549 (moderate expression, center), and HeLa (low expression, right) cells using ETS-1 (D8O8A) Rabbit mAb (green). Actin filaments were labeled with Dylight™ 554 Phalloidin #13054 (red). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 K-562 cells and either 10 μl of ETS-1 (D8O8A) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human c-Fos Promoter Primers #4663, human c-Myc intron 1 primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. | |
Western blot analysis of extracts from various cell lines using ETS-1 (D8O8A) Rabbit mAb. | |
Immunoprecipitation of ETS-1 protein from OVCAR8 cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or ETS-1 (D8O8A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using ETS-1 (D8O8A) Rabbit mAb |
