EXPAND1/MUM1 (D1Z6Y) Rabbit mAb
货号：
13437S 基本售价：
3360.0 元 规格：
-
产品信息
概述| 货号 | 13437S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 使用方法 | WB(1:1000) |
性能| 供应商 | CST |
| 背景 | DNA double-strand breaks (DSBs) are potentially hazardous lesions that can be induced by ionizing radiation (IR), radiomimetic chemicals, or DNA replication inhibitors. Melanoma associated antigen (mutated) 1 (MUM1, EXPAND1) is a PWWP-domain containing chromatin binding protein involved in maintaining chromatin architecture of interphase chromosomes. In response to DNA damage, EXPAND1/MUM1 accumulates at sites of DNA double strand breaks through direct interaction with DNA repair factor 53BP1 (1). Accumulation of EXPAND1/MUM1 at damaged DNA sites is thought to modify the structure of the chromatin and allow access to other DNA repair factors (2). 53BP1 activates the checkpoint kinase ATM and promotes DNA double strand break repair via nonhomologous end joining (NHEJ) repair pathway (3). DNA双链损伤（DSBs）是潜在的有害损伤区域可能由电离辐射（IR），拟辐射化合物，或DNA复制抑制因子所引发。黑色素瘤相关抗原（mutated）1（MUM1,EXPAND1）是一个含有PWWP-结构域的染色质结合蛋白，涉及了维持分裂中期染色体的染色质结构。当响应DNA损伤后，EXPAND1/MUM1通过直接和DNA损伤因子53BP1结合募集到DNA双链损伤区（1）。EXPAND1/MUM1募集到DNA损伤区被认为会修饰染色质的结构使其与其他DNA修复因子结合（2）。53BP1激发了检验点激酶ATM活性，促进DNA双链通过非同源末端连接（NHEJ）修复途径修复损伤（3）。 |
| 存放说明 | -20C |
| 计算分子量 | 85 |
参考图片Western blot analysis of extracts from various cell lines using EXPAND1/MUM1 (D1Z6Y) Rabbit mAb.使用EXPAND1/MUM1 (D1Z6Y) Rabbit mAb对多种细胞提取物进行western blot分析。 |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human EXPAND1/MUM1 (hEXPAND1/MUM1-Myc/DDK; +) protein, using EXPAND1/MUM1 (D1Z6Y) Rabbit mAb (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).模拟转染(-)或转染了表达Myc/DDK-tagged全长人EXPAND1/MUM1 (hEXPAND1/MUM1-Myc/DDK; +)蛋白的293T细胞提取物，使用EXPAND1/MUM1 (D1Z6Y) Rabbit mAb (上) 或 Myc-Tag (71D10) Rabbit mAb #2278 (下)进行western blot分析。 |
