| 货号 | 12565S |
| 反应种属 | Human/Mouse/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Exportin 5 (D7W6W) Rabbit mAb recognizes endogenous levels of total exportin 5 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Import and export through the nuclear envelope (NE) via facilitated translocation is important for many cellular processes including protein synthesis and miRNA biogenesis (1). Exportin 5 (XPO5) is a member of the importin β family of proteins (2) and functions in tRNA export in a sequence dependent fashion. More recently, it has been shown to export pre-miRNA by a RanGTPase-driven process from the nucleus to the cytoplasm, where pre-miRNA processing occurs to produce mature miRNAs (1,3). Study of the miRNA biosynthesis pathway is essential toward understanding the process of oncogenesis as global downregulation of miRNAs and the resulting alterations in expression of tumor suppressor and oncogenic proteins is a common phenotype of cancers cells (3,4). Research studies have shown that disruption of exportin 5 functions in many types of cancers including breast and lung, where pre-miRNA accumulates in the nucleus and miRNA maturation is impaired (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 136 |
Western blot analysis of extracts from various cell lines using Exportin 5 (D7W6W) Rabbit mAb. Western blot方法检测多种细胞系的提取物，使用的抗体为Exportin 5 (D7W6W) Rabbit mAb兔单抗。 | |
Immunoprecipitation of exportin 5 from HPB-ALL cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Exportin 5 (D7W6W) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Exportin 5 (D7W6W) Rabbit mAb. 免疫沉淀法检测 HPB-ALL 细胞提取物中的exportin 5，使用抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900（泳道2）或 Exportin 5 (D7W6W) Rabbit mAb兔单抗 (泳道3)。泳道1为10% input对照。 |
