| 货号 | 3147S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IF-IC |
| 目标/特异性 | Ezh2 (AC22) Mouse mAb detects endogenous levels of total Ezh2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). |
| 存放说明 | -20C |
| 计算分子量 | 98 |
Western blot analysis of extracts from various cell lines using Ezh2 (AC22) Mouse mAb. 使用Ezh2 (AC22) Mouse mAb鼠单抗，免疫印迹(Western blot)分析不同细胞系中Ezh2 (AC22)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using Ezh2 (AC22) Mouse mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). 使用Ezh2 (AC22) Mouse mAb 鼠单抗(绿色)标记，共聚焦免疫荧光分析HeLa细胞。Alexa Fluor® 555 phalloidin标记微丝蛋白(红色)。 |
