| 货号 | 13009S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 125 |
Immunoprecipitation of FAK from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or FAK (D2R2E) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FAK (D2R2E) Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) 或 FAK (D2R2E) Rabbit mAb (lane 3)对U-87 MG细胞提取物中的FAK进行免疫沉淀实验。Lane1是10%上样量。使用FAK (D2R2E) Rabbit mAb进行western blot分析。 | |
Western blot analysis of extracts from various cell lines using FAK (D2R2E) Rabbit mAb.使用FAK (D2R2E) Rabbit mAb对多种细胞提取物进行western blot分析。 |
