| 货号 | 4233S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IHC-P |
| 目标/特异性 | Fas (C18C12) Rabbit mAb detects endogenous levels of total human Fas protein. |
| 使用方法 | WB(1:1000) IHC-P (1:480) |
| 供应商 | CST |
| 背景 | Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9). |
| 存放说明 | -20C |
| 计算分子量 | 40-50 |
Western blot analysis of extracts from COS cells, mock transfected or transfected with human Fas, and from ACHN and HT-1080 cell lines using Fas (C18C12) Rabbit mAb. Western blot方法检测COS细胞提取物，空质粒或人Fas转染；以及 ACHN 和 HT-1080细胞系提取物，使用抗体为Fas (C18C12) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffn embedded human colon using Fas (C18C12) Rabbit mAb in the presence of control peptide (left) or antigen specific peptide (right). 免疫组织化学分析石蜡包埋的人结肠癌切片，对照肽（左）或抗原特异性肽（右），使用抗体为 Fas (C18C12) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Fas (C18C12) Rabbit mAb. 免疫组织化学分析石蜡包埋的人结肠癌切片，使用抗体为 Fas (C18C12) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded Raji (positive, left) or K562 cells (negative, right) using Fas (C18C12) Rabbit mAb. 免疫组织化学分析石蜡包埋的Raji (+；左)或K562(-；右) 细胞，使用抗体为 Fas (C18C12) Rabbit mAb兔单抗。 |
