| 货号 | 11835S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | FGF Receptor 2 (D4H9) Rabbit mAb recognizes endogenous levels of total FGF receptor 2 protein. This antibody does not cross-react with other FGF receptor family members. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Fibroblast growth factors (FGFs) produce mitogenic and angiogenic effects in target cells by signaling through cell surface receptor tyrosine kinases. There are four members of the FGF receptor family: FGFR1 (flg), FGFR2 (bek, KGFR), FGFR3, and FGFR4. Each receptor contains an extracellular ligand binding domain, a transmembrane domain, and a cytoplasmic kinase domain (1). Following ligand binding and dimerization, the receptors are phosphorylated at specific tyrosine residues (2). Seven tyrosine residues in the cytoplasmic tail of FGFR1 can be phosphorylated: Tyr463, 583, 585, 653, 654, 730, and 766. Tyr653 and Tyr654 are important for catalytic activity of activated FGFR and are essential for signaling (3). The other phosphorylated tyrosine residues may provide docking sites for downstream signaling components such as Crk and PLCγ (4,5). |
| 存放说明 | -20C |
| 计算分子量 | 92, 145 |
Western blot analysis of extracts from various cell lines using FGF Receptor 2 (D4H9) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测多个细胞系的提取物，使用的抗体为FGF Receptor 2 (D4H9) Rabbit mAb (上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图). | |
Western blot analysis of recombinant FGF Receptor 1, FGF Receptor 2, FGF Receptor 3, and FGF Receptor 4, as indicated, using FGF Receptor 2 (D4H9) Rabbit mAb (upper) or FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740 (lane 1), FGF Receptor 2 (D4H9) Rabbit mAb (lane 2), FGF Receptor 3 (C51F2) Rabbit mAb #4574 (lane 3), and FGF Receptor 4 (D3B12) XP® Rabbit mAb #8562 (lane 4) (lower).Western blot方法检测如下重组FGF受体1，FGF受体2，FGF受体3和FGF受体4，使用的抗体为FGF Receptor 2 (D4H9) Rabbit mAb (上图)或FGF Receptor 1 (D8E4) XP® Rabbit mAb #9740 (泳道1), FGF Receptor 2 (D4H9) Rabbit mAb (泳道2), FGF Receptor 3 (C51F2) Rabbit mAb #4574 (泳道3)和FGF Receptor 4 (D3B12) XP® Rabbit mAb #8562 (泳道4) (下图). | |
Western blot analysis of extracts from KATO III cells, transfected with 100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® FGF Receptor 2 siRNA I #12600 (+), using FGF Receptor 2 (D4H9) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).Western blot方法检测KATO III细胞提取物，细胞用100 nM SignalSilence® Control siRNA (Unconjugated) #6568 (-) or SignalSilence® FGF Receptor 2 siRNA I #12600 (+)转染，使用的抗体为FGF Receptor 2 (D4H9) Rabbit mAb (上图)或β-Actin (D6A8) Rabbit mAb #8457 (下图). |
