| 货号 | 12210S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | FKBP5 (D5G2) Rabbit mAb recognizes endogenous levels of total FKBP5 protein. This antibody does not cross-react with FKBP4 protein. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | FK506 binding protein 51 (FKBP51, also called FKBP5) belongs to the FKBP family of immunophilins (1). FKBP family proteins contain FK domains and TPR (tetratricopeptide repeat) domains. The FK domains are responsible for PPIase (peptidylprolyl isomerase) acitivity and allow binding to FK506 and rapamycin (2,3). The C terminal TPR domains are involved in protein-protein interactions. The TPR domain of FKBP5 mediates binding to HSP90 complexes (4), as well as glucocorticoid, androgen, and progesterone receptors, which account for its regulatory role in steroid hormone receptor function (5). FKBP5 also binds to IKKα and is involved in NF-κB signaling (6,7). In addition, FKBP5 was identified as a negative regulator of Akt, through promotion of Akt - PHLPP interaction and enhanced dephosphorylation of Akt (8). |
| 存放说明 | -20C |
| 计算分子量 | 51 |
Western blot analysis of extracts from HT-1080 and HeLa cells using FKBP5 (D5G2) Rabbit mAb. Western blot方法检测 HT-1080 和 HeLa细胞的提取物，使用的抗体为FKBP5 (D5G2) Rabbit mAb兔单抗。 | |
Immunoprecipitation of FKBP5 from HT-1080 cell extracts, using Normal Rabbit IgG #2729 (lane 2) or FKBP5 (D5G2) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using FKBP5 (D5G2) Rabbit mAb. 免疫沉淀法检测 HT-1080细胞提取物中的 FKBP5，使用抗体为Normal Rabbit IgG #2729 （泳道2）或 FKBP5 (D5G2) Rabbit mAb兔单抗 (泳道3)。泳道1为10% input对照。 Western blot 检测中使用的是 FKBP5 (D5G2) Rabbit mAb兔单抗。 |
