| 货号 | 8510S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | FLIP (D16A8) Rabbit mAb recognizes endogenous levels of total FLIP protein. This antibody also detects the short isoform of FLIP, FLIPS. The antibody detects a band of unknown origin at around 70 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Cellular FLIP (FLICE inhibitory protein) is a regulator of apoptosis identified in different groups and has various names such as c-FLIP (1), Casper (2), CLARP (3), FLAME (4), I-FLICE (5), MRIT (6), CASH (7), and Usurpin (8). FLIP is expressed as two alternative splice forms, FLIP short (FLIPS) and FLIP long (FLIPL). FLIPS contains two death effector domains (DEDs) found on the death receptor adaptor protein FADD and the pro-domain of caspase-8. FLIPL shares significant homology with caspase-8 (FLICE) and contains an additional death effector domain, but FLIPL lacks the catalytic active site of the caspases and does not have protease activity. Both forms have been reported to interact with FADD and pro-caspase-8. The role of FLIP in apoptosis is controversial as some have reported it to be anti-apoptotic, while others claim that it is pro-apoptotic. Overexpression of FLIPL can lead to caspase-8 heterodimers that produce an active protease resulting in apoptosis. However, at physiological levels, it is thought that the binding of FLIP to the DED of FADD results in inhibition of caspase-8 processing. Reduction of FLIP by siRNA or gene targeting sensitizes cells to death receptor-mediated apoptosis. FLIP has also been implicated in the resistance of cancer cells to apoptosis and is upregulated in some cancer types including Hodgkins lymphoma, and ovarian and colon carcinomas (9). |
| 存放说明 | -20C |
| 计算分子量 | 55 |
Western blot analysis of extracts from various cell lines using FLIP (D16A8) Rabbit mAb. Western blot 检测多种细胞系提取物，使用抗体为FLIP (D16A8) Rabbit mAb兔单抗。 | |
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with human FLIP (hFLIP, +), using FLIP (D16A8) Rabbit mAb. Western blot方法检测293T细胞提取物，空质粒（-）或人FLIP (hFLIP, +)转染；使用抗体为FLIP (D16A8) Rabbit mAb兔单抗。 |
