| 货号 | 3462S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | FLT3 (8F2) Rabbit mAb detects endogenous levels of FLT3 protein. The antibody does not cross-react with related proteins. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) |
| 供应商 | CST |
| 背景 | FMS-related tyrosine kinase 3 (FLT3, also called Flk2), is a member of the type III receptor tyrosine kinase family, which includes c-Kit, PDGFR and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream targets (3). The p85 subunit of PI3 kinase, SHP2, GRB2 and Shc are associated with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7). |
| 存放说明 | -20C |
| 计算分子量 | 130 nonglycosylated form;160 glycosylated mature form |
Western blot analysis of extracts from Baf3/FLT3 transfected cells and SEM leukemia cells, using FLT3 (8F2) Rabbit mAb.Western blot检测转染了Baf3/FLT3的细胞和SEM细胞的提取物，使用的抗体为：FLT3 (8F2) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded SEM cells, using FLT3 (8F2) Rabbit mAb.免疫组化检测石蜡包埋的SEM细胞，使用的抗体为：FLT3 (8F2) Rabbit mAb。 |
