| 货号 | 2880S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC/F |
| 目标/特异性 | FoxO1 (C29H4) Rabbit mAb detects endogenous levels of total FoxO1 protein. The antibody does not detect exogenously expressed family members FoxO3a or FoxO4. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:100) F (1:200) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 78 to 82 |
Western blot analysis of extracts from IGROV-1 and COS-7 cells using FoxO1 (C29H4) Rabbit mAb. | |
Western blot检测分析IGROV-1 和 COS-7细胞提取物。使用抗体是FoxO1 (C29H4) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded SKOV-3 xenograft using FoxO1 (C29H4) Rabbit mAb. 免疫组织化学方法检测石蜡包埋的SKOV-3异种移植物，使用的抗体是FoxO1 (C29H4) Rabbit mAb兔单抗。 | |
Immunohistochemical analysis of paraffin-embedded IGROV-1 cell pellets, LY294002-treated (left) or insulin-treated (right), using FoxO1 (C29H4) Rabbit mAb. Note the cytoplasmic localization of FoxO1 upon Akt activation. | |
免疫组织化学方法检测石蜡包埋的IGROV-1细胞颗粒，LY294002处理的 (左图) or insulin处理的 (右图)，使用的抗体是FoxO1 (C29H4) Rabbit mAb兔单抗。注意Akt激活后FoxO1蛋白的胞质定位。 | |
Confocal immunofluorescent analysis of IGROV-1 cells, LY294002-treated (left) or insulin-treated (right), using FoxO1 (C29H4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye). 激光共聚焦荧光法检测 IGROV-1细胞，左图为LY294002处理的，右图为insulin处理的，使用的抗体是FoxO1 (C29H4) Rabbit mAb兔单抗（绿色）。微丝用DY-554 phalloidin标记（红色）。蓝色伪彩是 DRAQ5™ (fluorescent DNA dye)。 |
