| 货号 | 2497S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | FoxO3a (75D8) Rabbit mAb detects exogenous and endogenous levels of total FoxO3a protein. The antibody does not detect the exogenously expressed family members FoxO4 or FoxO1. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 82 to 97 |
Western blot analysis of extracts from Jurkat and PC3 cells using FoxO3a (75D8) Rabbit mAb. Western blot检测分析Jurkat 和 PC3细胞提取物。使用抗体是FoxO3a (75D8) Rabbit mAb兔单抗。 | |
Confocal immunofluorescent analysis of SH-SY5Y cells, IGF-I treated (left) or LY294002-treated, using FoxO3a (75D8) Rabbit mAb (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 激光共聚焦荧光法检测SH-SY5Y细胞，左图为 IGF-I处理的，右图为LY294002处理的，使用的抗体是FoxO3a (75D8) Rabbit mAb兔单抗（绿色）。微丝用Alexa Fluor® 555 phalloidin标记（红色）。蓝色伪彩是 DRAQ5® #4084 (fluorescent DNA dye)。 | |
Western blot analysis of extracts from wild type (+/+) and FoxO3 knock-out (-/-) mouse brain, using FoxO3a (75D8) Rabbit mAb (upper) and FoxO1 (C29H4) Rabbit mAb #2880 (lower). (FoxO3 knock-out (-/-) mouse brain was kindly provided by Dr. Ron DePinho, Dana-Farber Cancer Institute, Boston, MA). Western blot检测分析野生型(+/+)和FoxO3 敲除型(-/-)小鼠脑提取物，使用的抗体是FoxO3a (75D8) Rabbit mAb 兔单抗(上图) and FoxO1 (C29H4) Rabbit mAb兔单抗 #2880 (下图)。(FoxO3 敲除型 (-/-) 小鼠脑由Dr. Ron DePinho, Dana-Farber Cancer Institute, Boston, MA，友情赠送)。 |
