| 货号 | 12829S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | FoxO3a (D19A7) Rabbit mAb recognizes endogenous levels of total FoxO3a protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:3200) F (1:400) IF-IC (1:800) |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 82 to 97 |
Immunohistochemical analysis of paraffin-embedded metastatic SKOV3 tumor in mouse lung using FoxO3a (D19A7) Rabbit mAb. Note nuclear staining in adjacent normal lung. 使用FoxO3a (D19A7) Rabbit mAb兔单抗对石蜡包埋的小鼠肺转移的SKOV3肿瘤组织进行免疫组化分析。注意相连的正常肺组织细胞核染色结果。 | |
Immunohistochemical analysis of paraffin-embedded PC-3 (upper) and MRK-nu-1 (lower) cell pellets, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb. 使用FoxO3a (D19A7) Rabbit mAb兔单抗对经过Human Insulin-like Growth Factor I (hIGF-I) #8917（左）或LY294002 #9901（右）处理后石蜡包埋的PC-3（上）和MRK-nu-1（下）进行免疫组化分析。 | |
Western blot analysis of extracts from 293T, MRK-nu-1 and Jurkat cells using FoxO3a (D19A7) Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). 使用FoxO3a (D19A7) Rabbit mAb兔单抗（上）和Akt (pan) (C67E7) Rabbit mAb兔单抗 #4691（下）对293T， MRK-nu-1 和 Jurkat进行western blot分析。 | |
Confocal immunofluorescent analysis of PC-3 cells, treated with Human Insulin-like Growth Factor I (hIGF-I) #8917 (left) or LY294002 #9901 (right), using FoxO3a (D19A7) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). 使用FoxO3a (D19A7) Rabbit mAb兔单抗对经过Human Insulin-like Growth Factor I (hIGF-I) #8917（左）或LY294002 #9901（右）处理（绿色）的PC-3细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝用鬼笔环肽标记（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using FoxO3a (D19A7) Rabbit mAb. 使用FoxO3a (D19A7) Rabbit mAb兔单抗对石蜡包埋的人乳腺癌组织进行免疫组化分析。 | |
Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using FoxO3a (D19A7) Rabbit mAb. 使用FoxO3a (D19A7) Rabbit mAb兔单抗对石蜡包埋的人前列腺癌组织进行免疫组化分析。 | |
Flow cytometric analysis of MCF7 cells (green) and MRK-NU-1 cells (blue) using FoxO3a (D19A7) Rabbit mAb. Anti-rabbit (H+L), F(ab)2 Fragment (PE Conjugate) #8885 was used as a secondary antibody. |
