| 货号 | 2499S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | The FoxO4 Antibody detects endogenous levels of total FoxO4 protein. The antibody also cross-reacts with an unidentified protein at 95 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The Forkhead family of transcription factors is involved in tumorigenesis of rhabdomyosarcoma and acute leukemias (1-3). Within the family, three members (FoxO1, FoxO4, and FoxO3a) have sequence similarity to the nematode orthologue DAF-16, which mediates signaling via a pathway involving IGFR1, PI3K, and Akt (4-6). Active forkhead members act as tumor suppressors by promoting cell cycle arrest and apoptosis. Increased expression of any FoxO member results in the activation of the cell cycle inhibitor p27 Kip1. Forkhead transcription factors also play a part in TGF-β-mediated upregulation of p21 Cip1, a process negatively regulated through PI3K (7). Increased proliferation results when forkhead transcription factors are inactivated through phosphorylation by Akt at Thr24, Ser256, and Ser319, which results in nuclear export and inhibition of transcription factor activity (8). Forkhead transcription factors can also be inhibited by the deacetylase sirtuin (SirT1) (9). |
| 存放说明 | -20C |
| 计算分子量 | 65 |
Western blot analysis of extracts from Jurkat and U937 cells using Fox04 Antibody. Western blot方法检测分析Jurkat 和 U937细胞提取物，使用的抗体是Fox04抗体。 |
