| 货号 | 19967S |
| 反应种属 | Human |
| 来源宿主 | Rabbit IgG |
| 应用 | W/IP/IHC-P/ChIP |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:7500) ChIP (1:50) |
| 供应商 | CST |
| 背景 | The Fos family of nuclear oncogenes includes c-Fos, FosB, Fos-related antigen 1 (FRA1), and Fos-related antigen 2 (FRA2) (1). While most Fos proteins exist as a single isoform, the FosB protein exists as two isoforms: full-length FosB and a shorter form, FosB2 (Delta FosB), which lacks the carboxy-terminal 101 amino acids (1-3). The expression of Fos proteins is rapidly and transiently induced by a variety of extracellular stimuli including growth factors, cytokines, neurotransmitters, polypeptide hormones, and stress. Fos proteins dimerize with Jun proteins (c-Jun, JunB, and JunD) to form Activator Protein-1 (AP-1), a transcription factor that binds to TRE/AP-1 elements and activates transcription. Fos and Jun proteins contain the leucine-zipper motif that mediates dimerization and an adjacent basic domain that binds to DNA. The various Fos/Jun heterodimers differ in their ability to transactivate AP-1 dependent genes. In addition to increased expression, phosphorylation of Fos proteins by Erk kinases in response to extracellular stimuli may further increase transcriptional activity (4-6). Phosphorylation of c-Fos at Ser32 and Thr232 by Erk5 increases protein stability and nuclear localization (5). Phosphorylation of FRA1 at Ser252 and Ser265 by Erk1/2 increases protein stability and leads to overexpression of FRA1 in cancer cells (6). Following growth factor stimulation, expression of FosB and c-Fos in quiescent fibroblasts is immediate, but very short-lived, with protein levels dissipating after several hours (7). FRA1 and FRA2 expression persists longer, and appreciable levels can be detected in asynchronously growing cells (8). Deregulated expression of c-Fos, FosB, or FRA2 can result in neoplastic cellular transformation; however, Delta FosB lacks the ability to transform cells (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 35-45 |
Immunohistochemical analysis of paraffin-embedded T-47D (Fra2 low expression, left) and U-2 OS (Fra2 high expression, right) cell pellets using Fra2 (D2F1E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Fra2 (D2F1E) Rabbit mAb. | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Fra2 (D2F1E) Rabbit mAb. | |
Western blot analysis of extracts from serum-starved HeLa cells, untreated (-) or stimulated with TPA #4174 (+) for 4 hr, using Fra2 (D2F1E) Rabbit mAb (upper) and α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). | |
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 Jurkat cells and either 10 μl of Fra2 (D2F1E) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using human CCR4 promoter primers, SimpleChIP® Human Sox4 Promoter Primers #32943, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one. |
