| 货号 | 5266S |
| 反应种属 | Human |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Frizzled5 (D2H2) Rabbit mAb detects endogenous levels of total Frizzled5 protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 50-65 |
Western blot analysis of extracts from HepG2 and 293 cells using Frizzled5 (D2H2) RmAb.使用Frizzled5 （D2H2）兔源单克隆抗体多HepG2和293 细胞提取物进行Western blot分析。 | |
Western blot analysis of extracts from COS cells, mock transfected or transfected with human Frizzled5, using Frizzled5 (D2H2) RmAb.人Frizzled5转染或未转染的COS细胞提取物进行Western blot实验，使用Frizzled5（D2H2）兔源单克隆抗体检测。 |
