| 货号 | 5158S |
| 反应种属 | Human/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | Frizzled6 (D16E5) Rabbit mAb detects endogenous levels of total Frizzled6 protein. |
| 使用方法 | WB(1:1000) IP (1:200) |
| 供应商 | CST |
| 背景 | Frizzled (Fzd) belongs to the seven transmembrane-spanning G-protein-coupled receptor (GPCR) superfamily (1). Fzds have a large extracellular N-terminal region containing a cysteine-rich domain (CRD), which is involved in binding to Wnt proteins (1,2). The intracellular C-terminus binds to the PDZ domain of Dvl proteins, a major signaling component downstream of Fzd (3). Wnt proteins bind to Fzd and the co-receptors LRP5 or LPR6, and activate Wnt/β-catenin pathway through inhibiting phosphorylation of β-catenin by GSK3-β (4,5). In addition to this canonical Wnt/β-catenin pathway, some Wnt proteins can also activate the Fzd/Ca2+ pathway and Fzd/PCP (planar cell polarity) pathway (6,7). The mammalian Fzd subfamily has 10 members (Fzd1 to Fzd10) and they may mediate signaling through different pathways (8). Some Fzds can also bind to other secreted proteins, like Norrin and R-Spondin (9-11). |
| 存放说明 | -20C |
| 计算分子量 | 75-85 |
Western blot analysis of extracts from 786-0, HeLa, and HCT 116 cells using Frizzled6 (D16E5) Rabbit mAb.使用Frizzled6（D16E5）兔源单克隆抗体对786-0, HeLa, 和HCT 116 细胞提取物进行Western blot分析。 | |
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Frizzled6 siRNA I #6596, 100 nM SignalSilence® Frizzled6 siRNA II #6597, or mock transfected, using Frizzled6 (D16E5) Rabbit mAb (upper) or β-Tubulin (9F3) Rabbit mAb #2128 (lower). The Frizzled6 (D16E5) Rabbit mAb confirms silencing of Frizzled6 expression, while the β-Tubulin (9F3) Rabbit mAb is used as a loading control.100 nM SignalSilence® Frizzled6 siRNA I #6596, 100 nM SignalSilence® Frizzled6 siRNA II #6597 转染或未转染的HeLa细胞提取物进行western blot实验，用Frizzled6 (D16E5)兔源单克隆抗体（上）或β-Tubulin (9F3) 兔源单克隆抗体 #2128（下）进行检测。Frizzled6 (D16E5)兔源单克隆抗体用于确认Frizzled6的敲除，β-Tubulin (9F3)兔源单克隆抗体用于上样参照物。 |
