| 货号 | 7098S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IF-IC |
| 目标/特异性 | FXR2 (D85D6) Rabbit mAb recognizes endogenous levels of total FXR2 protein. |
| 使用方法 | WB(1:1000) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation (1). X-linked FMRP (FMR-1) and its two autosomal homologs, FXR1 and FXR2, are polyribosome-associated RNA-binding proteins that are involved in the pathogenesis of fragile X syndrome (1-3). Each of the fragile X proteins can self-associate as well as form heteromers with the other two related proteins (3). FMRP can act as a translation regulator and is a component of RNAi effector complexes (RISC), suggesting a role in gene silencing (4). The Drosophila homolog of FMRP (dFMRP) associates with Argonaute 2 (Ago2) and Dicer and can coimmunoprecipitate with miRNA and siRNA (5). These results suggest that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. In addition, FMRP, FXR1 and FXR2 are components of stress granules (SG) and have been implicated in the translational regulation of mRNAs (6). |
| 存放说明 | -20C |
| 计算分子量 | 95 |
Confocal immunofluorescent analysis of C2C12 cells, untreated (left) or MG132-treated (10 μg/mL, 3 hr, right) using FXR2 (D85D6) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 使用FXR2 (D85D6) Rabbit mAb兔单抗（绿色），共聚焦免疫荧光观察TIAR蛋白在C2C12细胞中定位，细胞分为对照组 (左图)和MG132处理组(10 μg/mL, 3 小时，右图)。DY-554 phalloidin标记微丝蛋白（红色）。蓝色伪彩= DRAQ5® #4084 (DNA 荧光染料)。 | |
Western blot analysis of extracts from various cell lines using FXR2 (D85D6) Rabbit mAb. FXR2 (D85D6) Rabbit mAb兔单抗，Western Blotting分析不同细胞系中FXR2蛋白水平。 |
