| 货号 | 4076S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W |
| 目标/特异性 | GEF-H1 (55B6) Rabbit mAb detects endogenous levels of total GEF-H1 protein. |
| 使用方法 | WB(1:1000) |
| 供应商 | CST |
| 背景 | Rho family small GTPases regulate processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of GDP for GTP. GEF-H1 is a Rho GEF that localizes to microtubules and regulates Rho activity in response to microtubule destabilization (1). Loss of interaction between GEF-H1 and microtubules leads to activation of Rho (2). PAK1 phosphorylates GEF-H1 at Ser885, a site located in the 14-3-3 binding motif. Phosphorylation at this site is required for 14-3-3 binding and correlated to recruitment of 14-3-3 and GEF-H1 to microtubules (3). GEF-H1 has also been shown to localize to tight junctions and modulate polarized cell permeability (4,5). GEF-H1 is inactivated by binding to cingulin at epithelial tight junctions, inactivating RhoA and leading to G1/S arrest (5). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from various cell types, using GEF-H1 (55B6) Rabbit mAb. 使用GEF-H1 (55B6) Rabbit mAb，免疫印迹（Western Blot）分析不同细胞中GEF-H1 (55B6)蛋白水平。 | |
Immunoprecipitation of GEF-H1 from HeLa cell extracts, using GEF-H1 (55B6) Rabbit mAb. Western blot was performed using the same antibody. 使用GEF-H1 (55B6) Rabbit mAb，免疫沉淀分析HeLa细胞提取物的GEF-H1蛋白。使用同样的抗体进行免疫印迹（Western Blot）实验。 |
