| 货号 | 3670S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Mouse |
| 应用 | W/IHC-P/IF-F/IF-IC |
| 目标/特异性 | GFAP (GA5) Mouse mAb detects endogenous levels of total GFAP protein. |
| 使用方法 | WB(1:1000) IHC-P (1:50) IF-F (1:300) IF-IC (1:300) |
| 供应商 | CST |
| 背景 | The cytoskeleton consists of three types of cytosolic fibers: microfilaments (actin filaments), intermediate filaments and microtubules. Major types of intermediate filaments are specifically expressed in particular cell types: cytokeratins in epithelial cells, glial fibrillary acidic protein (GFAP) in glial cells, desmin in skeletal, visceral and certain vascular smooth muscle cells, vimentin in cells of mesenchymal origin and neurofilaments in neurons. GFAP and vimentin form intermediate filaments in astroglial cells and modulate their motility and shape (1). In particular, vimentin filaments are present at early developmental stages, while GFAP filaments are characteristic of differentiated and mature brain astrocytes. Thus, GFAP is commonly used as a marker for intracranial and intraspinal tumors arising from astrocytes (2). In addition, GFAP intermediate filaments are also present in non-myelin forming Schwann cells in the peripheral nervous system (3). |
| 存放说明 | -20C |
| 计算分子量 | 50 |
Western blot analysis of extracts from mouse and rat brain, using GFAP (GA5) Mouse mAb. Western blot分析大鼠和小鼠大脑提取物，使用的抗体是GFAP (GA5) Mouse mAb 鼠单抗。 | |
Confocal immunofluorescence image of rat hippocampus labeled with GFAP (GA5) Mouse mAb (red), Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854 (green), and CREB (48H2) Rabbit mAb #9197 (blue). | |
共聚焦免疫荧光分析大鼠大脑，使用GFAP (GA5) Mouse mAb 鼠单抗（红色）和Phospho-S6 Ribosomal Protein (Ser235/236) (2F9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4854（绿色）和CREB (48H2) Rabbit mAb 兔单抗 #9197（蓝色）进行标记。 |
