| 货号 | 8027S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 目标/特异性 | GGA3 (D66F1) Rabbit mAb recognizes endogenous levels of total GGA3 protein. This antibody may also recognize cross-reacting bands of unknown origin between 25 and 32 kDa. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | GGA3 is a member of the GGA family of proteins which also includes GGA1 and GGA2. These proteins consist of four distinct segments: a VHS domain that binds the di-leucine sorting signal DXXLL; a GAT domain that binds Arf-GTP; a hinge region that recruits clathrin; and a GAE domain that has sequence similarity to γ-adaptin and recruits a number of proteins. Arf1-GTPase recruits GGA3 to the trans-Golgi network. GGAs sort acid hydrolases to the lysosome and are involved in transporting proteins containing the DXXLL signal from the Golgi complex to the endosome (1). During apoptosis or cerebral ischemia, GGA3 is cleaved by caspase-3 at Asp313, reducing GGA3 levels and lysosomal degradation of β-secretase (BACE). The resulting elevated amount and activity of BACE plays a role in amyloid-β (Aβ) production, consistent with BACE elevation and Aβ accumulation in Alzheimer’s Disease (2). |
| 存放说明 | -20C |
| 计算分子量 | 90 |
Western blot analysis of extracts from rat brain, NIH/3T3 cells, and mouse brain using GGA3 (D66F1) Rabbit mAb. Western blot分析大鼠大脑、NIH/3T3 细胞和小鼠大脑提取物，使用的抗体是GGA3 (D66F1)Rabbit mAb 兔单抗。 | |
Western blot analysis of extracts from H4 cells, untreated, transfected with human GGA3 (hGGA3), or transfected with hGGA3 and treated with Staurosporine #9953 (1 μM, 3 hrs), using GGA3 (D66F1) Rabbit mAb. Western blot分析H4细胞提取物，未处理或转染人GGA3 (hGGA3)或转染hGGA3并用1 μl Staurosporine #9953处理3小时，使用的抗体是GGA3 (D66F1) Rabbit mAb 兔单抗。 |
