| 货号 | 13192S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | <p>The Golgi-associated protein golgin A1 (GOLGA1, golgin-97) was first isolated as a Golgi complex autoantigen associated with the autoimmune disorder Sjogrens syndrome (1). The golgin-97 protein contains a carboxy-terminal GRIP domain and is a commonly used trans-Golgi network (TGN) marker. All four known mammalian GRIP domain-containing proteins (golgin-97, golgin-245, GCC88 and GCC185) are found in the TGN, share extensive alpha-helical structure, and form homodimers (2). While all four golgin proteins localize to the TGN, they exhibit different membrane-binding abilities and are found in distinct TGN regions (3). Golgin-97 and golgin-245 are targeted to the trans-Golgi network through an interaction between their GRIP domains and the Arl1 protein switch II region (4). Overexpression studies and siRNA assays with GRIP domain-containing proteins suggest that these proteins help to maintain trans-Golgi network integrity and function by controlling localization of TGN resident proteins (5). By using a Shiga toxin B fragment (STxB)-based in vitro transport assay and an E-cadherin transport model system, golgin-97 and its effector Arl1-GTP were shown to play a role in trans-Golgi endosomal trafficking (6,7). Research studies also suggest that golgin-97 may play a role in poxvirus morphogenesis and maturation (8,9).</p>高尔基体相关蛋白golgin A1 (GOLGA1, golgin-97)最早被认为是一个高尔基体复合物自身抗原，和自身免疫综合征Sjogrens疾病相关（1）。golgin-97有一个羧基端GRIP结构域，经常被作为trans-Golgi network (TGN)的靶标。所有已知的四个哺乳动物带GRIP结构域蛋白(golgin-97, golgin-245, GCC88 and GCC185)都在TGN被发现，共享了alpha-螺旋结构并形成了同二聚体（2）。当所有的四个golgin蛋白都定位到TGN后，它们占线了不同的细胞膜结合能力，并被发现在不同的TGN区域（3）。Golgin-97 和 golgin-245通过它们的GRIP结构域和Arl1蛋白switch II相互作用而定位在trans-Golgi (4)。对含有GRIP结构域的蛋白进行过表达和敲除实验发现这些蛋白帮助维持了trans-Golgi网络的一体性，并通过控制TGN残留蛋白的位置而发挥功能（5）。通过使用Shiga toxin B fragment (STxB)-based体外运输实验和E-cadherin转运模型体系，结果表明golgin-97和它的效应分子Arl1-GTP在trans-Golgi内体运输过程中发挥了作用（6,7）。研究也表明golgin-97可能参与了痘病毒的生产和成束（8,9）。 |
| 存放说明 | -20C |
| 计算分子量 | 97 |
Confocal immunofluorescent analysis of HeLa cells using Golgin-97 (D8P2K) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).使用Golgin-97 (D8P2K) Rabbit mAb (绿色)对HeLa细胞进行激光共聚焦免疫荧光分析。肌动蛋白丝使用DyLight™ 554 Phalloidin #13054(红色)标记。蓝色假色= DRAQ5®#4084 (fluorescent DNA dye). | |
Western blot analysis of extracts from SK-MEL-5, HeLa, and C2C12 cells using Golgin-97 (D8P2K) Rabbit mAb.使用Golgin-97 (D8P2K) Rabbit mAb对SK-MEL-5, HeLa,和C2C12细胞进行western blot分析。 | |
Immunoprecipitation of Golgin-97 from SK-MEL-5 cell extracts using Rabbit (DA1E) mAb IgG XP®Isotype Control #3900 (lane 2) or Golgin-97 (D8P2K)Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Golgin-97 (D8P2K)Rabbit mAb.使用Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或Golgin-97 (D8P2K) Rabbit mAb (lane 3)对SK-MEL-5细胞中的Golgin-97进行免疫沉淀实验。Lane1是10%上样量。使用Golgin-97 (D8P2K) Rabbit mAb.进行western blot分析。 |
