| 货号 | 5676S |
| 反应种属 | Human/Mouse/Rat/Hamster/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | GSK-3α/β (D75D3) XP® Rabbit mAb detects endogenous levels of total GSK-3α and GSK-3β. The peptide sequence used as the antigen is 100% conserved between GSK-3α and GSK-3β in humans, monkeys, mice and rats. The antibody may prefer the conformation of GSK-3β by IF-IC. |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3 kinase/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5). GSK-3α regulates the production of amyloid-β peptides, a major component of the plaques that accumulate with progression of Alzheimer disease. Administration of therapeutic concentrations of lithium, a GSK-3 inhibitor, attenuates amyloid-β production by specifically inhibiting the cleavage of amyloid precursor protein (APP) by γ-secretase, blocking accumulation of amyloid-β peptides in the brains of mice that overproduce APP (6). |
| 存放说明 | -20C |
| 计算分子量 | 51, 46 |
Western blot analysis of extracts from HeLa cells, mock transfected or transfected with SignalSilence® GSK-3α/β siRNA #6301, using GSK-3α/β (D75D3) XP® Rabbit mAb (upper) and Akt (pan) (C67E7) Rabbit mAb #4691 (lower). western blot 分析Hela细胞提取物，mock转染组或SignalSilence® GSK-3α/β siRNA #6301转染组，所用抗体为GSK-3α/β (D75D3) XP® Rabbit mAb兔单抗 (上) 和 Akt (pan) (C67E7) Rabbit mAb 兔单抗#4691 (下). | |
Western blot analysis of extracts from GSK-3β (-/-) (lanes 1,2), GSK-3α (-/-) (lanes 3,4) and wild type (lanes 5,6) mouse embryonic fibroblast cells (MEF), untreated or insulin treated, using GSK-3α/β (D75D3) XP® Rabbit mAb. (MEF wild type, GSK-3α (-/-) and GSK-3β (-/-) cells were kindly provided by Dr. Jim Woodgett, University of Toronto, Canada). Western blot 分析GSK-3β (-/-) (泳道 1,2), GSK-3α (-/-) (泳道 3,4) 和 wild type (泳道 5,6) 的小鼠胚胎成纤维细胞（MEF）的提取物，分为未刺激组和胰岛素刺激组，所用抗体为GSK-3α/β (D75D3) XP™ Rabbit mAb兔单抗. (MEF wild type, GSK-3α (-/-) 和 GSK-3β (-/-) 细胞是由加拿大多伦多大学Dr. Jim Woodgett友情提供). |
