| 货号 | 14957S |
| 反应种属 | Human/Mouse/Rat |
| 来源宿主 | Rabbit |
| 应用 | W/IP |
| 使用方法 | WB(1:1000) IP (1:50) |
| 供应商 | CST |
| 背景 | The voltage-activated ion channel superfamily includes both cyclic nucleotide-gated (CNG) channels and hyperpolarization-activated cyclic nucleotide-modulated (HCN) channels (1,2). The direct binding of the cyclic nucleotides cAMP and cGMP promotes the gating of these channels, from the closed to an open state. Research studies demonstrate that HCN channels are involved in repetitive firing, resting membrane potential, and dendritic integration in neurons (1,3). HCN proteins are found in homotetramers and heterotetramers composed of HCN1 and HCN2 subunits (4). Tetramerization of HCN proteins results from cAMP binding to the channel, which triggers the release of the tonic inhibition exerted by the cytoplasmic cyclic nucleotide binding domain on the channel pore (5). HCN channels expressed in dorsal root ganglia neurons may play a role in acute inflammatory pain (6). Cardiac HCN channels ensure electrical rhythmicity in cardiomyocytes (7). |
| 存放说明 | -20C |
| 计算分子量 | 120 |
Western blot analysis of extracts from various tissues using HCN2 (D1C6I) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). | |
Immunoprecipitation of HCN2 from mouse brain extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HCN2 (D1C6I) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HCN2 (D1C6I) Rabbit mAb. |
