| 货号 | 3949S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP/IF-IC |
| 目标/特异性 | HDAC3 (7G6C5) Mouse mAb detects endogenous levels of total HDAC3 protein. The antibody does not cross-react with other HDAC proteins. |
| 使用方法 | WB(1:1000) IP (1:100) IF-IC (1:100) |
| 供应商 | CST |
| 背景 | Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7). |
| 存放说明 | -20C |
| 计算分子量 | 49 |
Western blot analysis of extracts from various cell lines using HDAC3 (7G6C5) Mouse mAb. 使用HDAC3 (7G6C5) Mouse mAb鼠单抗，免疫印迹(Western blot)分析不同细胞中HDAC3 (7G6C5)的蛋白水平。 | |
Confocal immunofluorescent analysis of HeLa cells using HDAC3 (7G6C5) Mouse mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). 使用HDAC3 (7G6C5) Mouse mAb鼠单抗 (绿色)，共聚焦免疫荧光分析HeLa细胞。DY-554 Phalloidin标记微丝蛋白(红色)。 |
