| 货号 | 4044S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Mouse |
| 应用 | W/IP |
| 目标/特异性 | HEF1/NEDD9 (2G9) Mouse mAb detects endogenous levels of total HEF1/NEDD9 protein. |
| 使用方法 | WB(1:1000) IP (1:100) |
| 供应商 | CST |
| 背景 | Human enhancer of filamentation protein 1 (HEF1), also known as neural precursor cell expressed developmentally down-regulated protein 9 (NEDD9), is part of the Cas family of proteins, which include HEF1/NEDD9, p130Cas and Efs (1). HEF1 is a predominantly cytoplasmic protein, localizing to focal adhesions during interphase, and centrosomes and other parts of the mitotic apparatus during G2/M phase of the cell cycle (2). HEF1 is a docking protein that plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion, motility, growth and apoptosis (1). Phosphorylation of HEF1 is induced by a number of factors, including FAK, TGF-β, PDGFR, Abl, and BCR-ABL, which leads to coordinate binding of multiple downstream effector proteins via 15 known SH2 domain-binding sites (1). HEF1 is a key regulator of cancer metastasis. It is required for the invasive activity of glioblastomas (3), is linked to the promotion of metastasis in melanoma (4), and is found to be up-regulated in lung cancer metastasis (5). |
| 存放说明 | -20C |
| 计算分子量 | 105, 115 |
Western blot analysis of extracts from A549 and C2C12 cell lines using HEF1/NEDD9 (2G9) Mouse mAb. 使用HEF1/NEDD9 (2G9) Mouse mAb，免疫印迹（Western Blot）分析A549和C2C12细胞中HEF1/NEDD9 (2G9)蛋白水平。 |
