| 货号 | 2165S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IHC-F/IF-IC/F |
| 目标/特异性 | HER2/ErbB2 (29D8) Rabbit mAb detects endogenous levels of total ErbB2 protein. This antibody does not cross-react with related kinases. |
| 使用方法 | WB(1:1000) IP (1:100) IHC-P (1:400) F (1:200) IF-IC (1:200) IHC-F (1:400) |
| 供应商 | CST |
| 背景 | The ErbB2 (HER2) proto-oncogene encodes a 185 kDa transmembrane, receptor-like glycoprotein with intrinsic tyrosine kinase activity (1). While ErbB2 lacks an identified ligand, ErbB2 kinase activity can be activated in the absence of a ligand when overexpressed and through heteromeric associations with other ErbB family members (2). Amplification of the ErbB2 gene and overexpression of its product are detected in almost 40% of human breast cancers (3). Binding of the c-Cbl ubiquitin ligase to ErbB2 at Tyr1112 leads to ErbB2 poly-ubiquitination and enhances degradation of this kinase (4). ErbB2 is a key therapeutic target in the treatment of breast cancer and other carcinomas and targeting the regulation of ErbB2 degradation by the c-Cbl-regulated proteolytic pathway is one potential therapeutic strategy. Phosphorylation of the kinase domain residue Tyr877 of ErbB2 (homologous to Tyr416 of pp60c-Src) may be involved in regulating ErbB2 biological activity. The major autophosphorylation sites in ErbB2 are Tyr1248 and Tyr1221/1222; phosphorylation of these sites couples ErbB2 to the Ras-Raf-MAP kinase signal transduction pathway (1,5). |
| 存放说明 | -20C |
| 计算分子量 | 185 |
Western blot analysis of cell extracts from various cell lines, using HER2/ErbB2 (29D8) Rabbit mAb.Western blot法检测各种细胞系提取物，使用抗体为：HER2/ErbB2 (29D8) RmAb。 | |
Immunohistochemical analysis of paraffin-embedded SKBr3 (high HER2) (left), MDA-MB-453 (moderate HER2) (middle) and MDA-MB-468 (low HER2) (right), using HER2/ErbB2 (29D8) Rabbit mAb.免疫组化检测石蜡包埋的SKBr3 (高表达 HER2) (左), MDA-MB-453 (中表达HER2) (中) 和 MDA-MB-468 (低表达HER2) (右)，使用抗体为：HER2/ErbB2 (29D8) Rabbit mAb。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using HER2/ErbB2 (29D8) Rabbit mAb.免疫组化检测石蜡包埋的人乳腺癌组织，使用抗体为：HER2/ErbB2 (29D8) Rabbit mAb.。 | |
Flow cytometric analysis of MCF7 cells using HER2/ErbB2 (29D8) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).流式细胞术检测MCF7细胞，使用抗体为：HER2/ErbB2 (29D8) Rabbit mAb（蓝色），非特异结合阴性对照抗体（红色）。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HER2/ErbB2 (29D8) RmAb in the presence of control peptide (left) or HER2/ErbB2 Blocking Peptide #1059 (right).免疫组化检测石蜡包埋的人乳腺癌组织，使用抗体为：HER2/ErbB2 (29D8) RmAb。左图，使用对照多肽；右图，使用HER2/ErbB2 Blocking Peptide #1059。 | |
Immunohistochemical analysis of frozen SKOV-3 xenograft using HER2/ErbB2 (29D8) Rabbit mAb.免疫组化检测SKOV-3异种移植物冰冻组织，使用抗体为：HER2/ErbB2 (29D8) RmAb。 | |
Confocal immunofluorescent analysis of MDA-MB-453 cells (left) and MDA-MB-231 cells (right), using HER2/ErbB2 (29D8) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). |
