| 货号 | 11988S |
| 反应种属 | Human/Mouse/Rat/Monkey |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P |
| 目标/特异性 | HES1 (D6P2U) Rabbit mAb recognizes endogenous levels of total HES1 protein. |
| 使用方法 | WB(1:1000) IP (1:200) IHC-P (1:6400) |
| 供应商 | CST |
| 背景 | HES1 (Hairy and Enhancer of Split 1) is one of seven members of the HES family of basic helix-loop-helix (bHLH) transcription factors which function primarily to repress transcription of bHLH-dependent genes (1). HES1 is understood to play an important conserved role in maintaining pluripotency of embryonic and adult stem/progenitor cells via the transcriptional repression of genes that promote differentiation (1,2). HES1 is particularly well known as a repressive mediator of the canonical Notch signaling pathway (3). HES1 plays a key role in mediating Notch-dependent T cell lineage commitment (4), and has been reported to be an essential mediator of Notch-induced T cell acute lymphoblastic leukemia (T-ALL) (4,5). HES1 is also reported to mediate Notch-induced repression of differentiation in a number of cancer cell types. A conditional deletion of HES1 from intestinal tumor cells in APC-mutant mice reduced tumor cell proliferation, while promoting differentiation toward epithelial lineages (6). Overexpression of HES1 in a human osteosarcoma (OS) cell line was shown to repress expression of the Notch antagonist Dtx1, leading to increased OS cell invasiveness (7). Other genes subject to transcriptional repression by HES1 include Neurogenin-2, Math1/Atoh1 and the NOTCH ligands DLL1 and Jagged1 (6,8,9). |
| 存放说明 | -20C |
| 计算分子量 | 30 |
Western blot analysis of extracts from various cell lines using HES1 (D6P2U) Rabbit mAb. 使用HES1 (D6P2U)Rabbit mAb对多种细胞提取物进行western blot分析。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using HES1 (D6P2U) Rabbit mAb. 使用HES1 (D6P2U)Rabbit mAb对石蜡包埋的人乳腺癌组织进行激光共聚焦免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using HES1 (D6P2U) Rabbit mAb. 使用HES1 (D6P2U)Rabbit mAb对石蜡包埋的人肺癌组织进行激光共聚焦免疫荧光分析。 | |
Immunohistochemical analysis of paraffin-embedded CUTLL1 cell pellets, control (left) or Compound E-treated (right), using HES1 (D6P2U) Rabbit mAb. 使用HES1 (D6P2U)Rabbit mAb对石蜡包埋的CUTLL1细胞，对照（左）或Compound E-处理（右）进行免疫组化分析。 |
