HEXIM1 (D5Y5K) Rabbit mAb【科瑞奥博】

产品中心

当前位置：首页>产品中心

HEXIM1 (D5Y5K) Rabbit mAb

货号： 12604S 基本售价： 3360.0 元规格： 100ul

产品信息

概述
货号	12604S
反应种属	Human/Monkey
来源宿主	Rabbit
应用	W/IP/IF-IC
目标/特异性	HEXIM1 (D5Y5K) Rabbit mAb recognizes endogenous levels of total HEXIM1 protein.
使用方法	WB(1:1000) IP (1:100) IF-IC (1:1200)

性能
供应商	CST
背景	Hexamethylene bis-acetamide-inducible protein 1 (HEXIM1) was originally identified in vascular smooth muscle cells as a protein that is upregulated upon treatment with the differentiating agent hexamethylene bisacetamide (1). HEXIM1 binds 7SK RNA, a highly abundant non-coding RNA, and together they act as a potent inhibitor of positive transcription elongation factor b (P-TEFb) (2,3). P-TEFb phosphorylates the C-terminal domain of the largest subunit of RNA polymerase II and is an important regulator of transcription elongation (4-8). 7SK RNA-bound HEXIM1 interacts with the cyclin T1 subunit of P-TEFb, sequestering P-TEFb in an inactive form leading to transcription inhibition (2,3). The regulation of the relative ratio of inactive to active P-TEFb in the cell by HEXIM1/7SK RNA is thought to play a critical role in regulation of a wide range of cellular gene expression programs such as estrogen and glucocorticoid receptor regulated genes (9-12).
存放说明	-20C
计算分子量	60

参考图片

Confocal immunofluorescent analysis of HeLa cells using HEXIM1 (D5Y5K) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).采用共聚焦免疫荧光术检测HeLa细胞，使用的抗体为Di- HEXIM1 (D5Y5K) Rabbit mAb (绿色)。肌动蛋白微丝使用DY-554 phalloidin进行标记（红色）。

Western blot analysis of extracts from various cell lines using HEXIM1 (D5Y5K) Rabbit mAb.Western blot方法检测不同细胞系的提取物，使用的抗体为HEXIM1 (D5Y5K) Rabbit mAb。

Immunoprecipitation of HEXIM1 from HeLa cell extracts, using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or HEXIM1 (D5Y5K) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using HEXIM1 (D5Y5K) Rabbit mAb.从 HeLa细胞提取物中免疫沉淀HEXIM1，使用的抗体为Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2)或 HEXIM1 (D5Y5K) Rabbit mAb (lane 3)。Lane 1为10% input。使用HEXIM1 (D5Y5K) Rabbit mAb进行Western blot检测。