| 货号 | 2024S |
| 反应种属 | Human/Mouse |
| 来源宿主 | Rabbit |
| 应用 | W/IP/IHC-P/IF-IC |
| 目标/特异性 | Hexokinase I (C35C4) Rabbit mAb detects endogenous levels of total hexokinase I protein. |
| 使用方法 | WB(1:1000) IP (1:50) IHC-P (1:800) IF-IC (1:200) |
| 供应商 | CST |
| 背景 | Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with rapid cell growth and proliferation (2,3). |
| 存放说明 | -20C |
| 计算分子量 | 102 |
Western blot analysis of extracts from various cell types using Hexokinase I (C35C4) Rabbit mAb. 对多个细胞系抽提液使用Hexokinase I (C35C4) Rabbit mAb兔单抗进行Western blot分析。 | |
Confocal immunofluorescent analysis of HeLa cells using Hexokinase I (C35C4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye). 对HeLa细胞使用Hexokinase I (C35C4) Rabbit mAb兔单抗（绿色）进行共聚焦免疫荧光分析。肌动蛋白用DY-554 phalloidin（红色）标记。蓝色伪彩=DRAQ5?#4084(荧光DNA染料)。 | |
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Hexokinase I (C35C4) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right). 对石蜡包埋的人乳腺癌使用Hexokinase I (C35C4) Rabbit mAb进行免疫组织化学分析，其中左图为存在对照肽，右图为存在抗原特异性肽。 | |
Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Hexokinase I (C35C4) Rabbit mAb.对石蜡包埋的人肺癌使用Hexokinase I (C35C4) Rabbit mAb进行免疫组化分析。 |
